Activated Partial Thromboplastin Time - APTT/ PTTK)
Details
Description
Test Code: RHM10003
Method: Photo - Optical
Sample: 3 mL Whole blood in 1 Blue Top (Sodium Citrate) tube. Mix thoroughly by inversion. Transport to Lab within 4 hours. If this is not possible, make PPP within 1 hour of collection as follows: Centrifuge sample at 3600 rpm fo 15 min.& transfer supernatant to a clean plastic tube. Centrifuge this supernatant again at 3600 rpm for 15 min. & finally transfer the supernatant (PPP) to 1 labelled clean plastic screw capped vial. FREEZE IMMEDIATELY. Ship frozen. DO NOT THAW. Overnight fasting is preferred. Duly filled Coagulation Requisition Form (Form 15) is mandatory.
Technique: Local samples: 2-8 C to reach lab in 3 hours.
Outstation samples:(-20C)
Sample Collection Instruction: Preparation of PPP :
Centrifuge the blood sample at 3000rpm for 15 minutes within 1 hr. of collection to separate the plasma. Transfer the Plasma obtained to a clean plastic tube using plastic tipped pipette avoiding buffy coat & centrifuge it again at 3700 rpm for 10 minutes & finally transfer the PPP into fresh, clean & labelled plastic tubes which contains <10,000 cells/cu. mm. DULY FILLED COAGULATION REQUISITION TO BE ATTACHED
