Technique: For Local samples:
3 ml. Whole blood in 1 Sodium Citrate (Light Blue Top) tube.
For Outstation samples:
2 ml. (1.5 ml. Minimum) PPP (Platelet Poor Plasma) from 2 Sodium Citrate tubes prepared
within 1 hr of collection and 3 ml. (2 ml. Minimum) Whole Blood in 1 EDTA tube. Aliquot
PPP in 2 transport vials and FREEZE.
Technique: Local samples: 2-8 C to reach lab in 3 hours.
Outstation samples:(-20C)
Sample Collection Instruction: Preparation of PPP :
Centrifuge the blood sample at 3000 rpm for 15 minutes within 1 hr. of collection to separate the plasma. Transfer the Plasma obtained to a clean plastic tube using plastic tipped pipette
avoiding buffy coat & centrifuge it again at 3700 rpm for 10 minutes finally transfer the PPP into fresh, clean & labelled plastic tubes which contains <10,000 cells/cu. mm. DULY FILLED COAGULATION REQUISITION TO BE ATTACHED
