Technique: 4 ml. (3 ml. Minimum) PPP (Platelet Poor Plasma) from 2 Sodium Citrate tubes prepared within 1 hr of collection. Aliquot PPP in 2 transport vials and FREEZE.
Technique: (-20C) in dry ice.
Sample Collection Instruction: Preparation of PPP :
Centrifuge the blood sample at 3000rpm for 15 minutes within 1 hr. of collection to separate the plasma. Transfer the Plasma obtained to a clean plastic tube using a plastic tipped pipette avoiding buffy coat & centrifuge it again at 3700 rpm for 10 minutes finally transfer the PPP into fresh, clean & labelled plastic tubes which contains <10,000 cells/cu. mm.
