Technique: High Performance Liquid Chromatography (HPLC)
Technique: 50 ml. (10 ml. Minimum) well mixed aliquot of 24 Hour urine in a sterile screw capped plastic container. Collect urine with appropriate volume of 50% HCL (6N HCL) to maintain pH between1- 3. Do not use concentrated HCL. Record 24 hour volume on test requisition form & urine container.