Technique: 4 ml. (3 ml. Minimum) Platelet
Poor Plasma (PPP) from 2
Sodium Citrate (Light Blue Top) tubes along with duly filled coagulation requisition form.
Technique: (-20C) in dry ice.
Sample Collection Instruction: Preparation of PPP :
12 -14 Hrs. (Overnight) fasting is preferred. Centrifuge the blood sample at 3000rpm for 10 minutes within 1 hr. of collection to separate the plasma. Transfer the Plasma obtained to a clean plastic tube using plastic tipped pipette avoiding buffy coat & centrifuge it again at 3700 rpm & finally transfer the PPP into fresh, clean & labelled plastic tubes which contains <10,000 cells/cu. mm. DULY FILLED COAGULATION REQUISITION TO BE ATTACHED
