Prenatal Chromosomal Microarray Analysis (CMA) with MCC
Microarray is a high resolution technology capable of detecting microdeletions / microduplication syndromes associated with ultrasound abnormalities which cannot be detected by conventional karyotyping
Details
Description
Methodology: KaryoTrack is a chromosomal microarray analysis (CMA) test performed using single nucleotide polymorphism (SNP) probes. It consists of approximately 700K probes targeting more than 4800 genes and regions with known disease associations, allowing for the analysis of genetic variation across the genome. The samples are processed using the standard assay protocol, where 200 ng of genomic DNA is amplified overnight for whole-genome amplification. The amplified DNA is then enzymatically fragmented and subjected to precipitation. The resuspended DNA samples are hybridized to a chip, and the samples undergo labeling, washing, and staining. The chips are imaged using a scanner. The data generated from the assay will be analyzed using a bioinformatic tool on the Human reference genome (GRCh38/hg38).
Sample Collection Instruction:
Minimum 3ml of peripheral blood, 300-500mg of CVS; Amniotic fluid of 15-20ml/T25 culture flask with 100% confluency, Maternal blood sample to be sent along for MCC testing.
Should NOT FREEZE. Should NOT be placed in formalin
Reported on: 21st working Day by 7:00 p.m.
